このエントリーをはてなブックマークに追加
ID 21464
file
creator
Kuratsune, Masatoshi
Hirai, Takayuki
Kiribayashi, Kei
Yokoyama, Yukio
Arakawa, Tetsuji
Yorioka, Noriaki
subject
fibrosis
STAT3
UUO
NDC
Medical sciences
abstract
Background: In vitro studies suggest that the signal transducer and activator of transcription (STAT) plays a critical role in renal fibrosis. However, the process of STAT activation in vivo remains unclear. This study in rats aimed to localize STAT3 activation within the kidney and examine the in vivo relationship between STAT3 activation and renal fibrosis Methods: Unilateral ureteral obstruction (UUO) was induced in the rats and the kidneys examined 3 or 7 days after obstruction. Activation of STAT3 in western blot and immunohistochemical analyses was identified by the phosphorylated form of STAT3 (pSTAT3). Results: Myofibroblasts were identified by alpha-smooth muscle actin expression and were upregulated in obstructed kidneys. pSTAT3 was localized mainly in tubular epithelial cells of collecting ducts in normal and obstructed kidneys and interstitial cells in obstructed kidneys. After UUO, western blotting showed a fourfold increase in pSTAT3, with a peak at day 7. Immunostaining showed a sixfold increase in pSTAT3 at day 7 in tubular epithelial cells and a 2500-fold increase at day 7 in interstitial cells. Conclusion: STAT3 was activated in rat tubular epithelial cells and myofibroblasts after UUO, suggesting that STAT3 may contribute to the progression of interstitial fibrosis.
journal title
Nephrology
volume
Volume 12
issue
Issue 6
start page
565
end page
571
date of issued
2007-12
publisher
Blackwell Publishing
issn
1320-5358
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright(c) 2007 Blackwell Publishing
relation
The definite version is avairable at www.blackwell-synergy.com
relation is version of URL
http://dx.doi.org/10.1111/j.1440-1797.2007.00881.x
department
Graduate School of Biomedical Science