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ID 29242
file
creator
Yanase, Yuhki
Araki, Atsunori
Suzuki, Hidenori
Tsutsui, Tomoko
Kimura, Tatsuo
Okamoto, Keishi
Nakatani, Tatsuyuki
subject
Biosensor
Surface plasmon resonance
Living cell
Optical fiber
Mast cells
NDC
Medical sciences
abstract
Surface plasmon resonance (SPR) sensors provide a useful means to study the interactions of biological molecules and the reaction of living cells on a sensor chip. However, conventional SPR sensors are bulky. expensive and complicated to use as common diagnostic equipment. In this study, we developed a relatively small and simple SPR system. using optical fibers of 250 pm diameter to detect the activation of living cells attached to the fiber tip. For this system, the core of 200 pm diameter with I cm length of an optical fiber was coated by gold film with 50 nm thickness to cause plasmon resonance. The light provided by a white LED and attenuated due to a SPR phenomenon in the sensor part was detected and analyzed using a spectrum detector. The difference in solvents with various refractive indexes and protein-bindings to the sensor tip was detected with sufficient sensitivity. Moreover, it detected a sustained increase of AR in a real-time manner, when RBL-2H3 mast cells were fixed onto the fiber tip and stimulated by an antigen. This small fiber SPR system might serve as a useful toot for various clinical examinations either within or outside the body.
journal title
Biosensors & Bioelectronics
volume
Volume 25
issue
Issue 5
start page
1244
end page
1247
date of issued
2010-01-15
publisher
Elsevier Advanced Technology
issn
0956-5663
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2009 Elsevier B.V. All rights reserved.
relation url
department
Graduate School of Biomedical Science