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ID 20782
file
creator
Okabe, Tsutomu
Morita, Eishin
Koro, Osamu
Yamamoto, Shoso
subject
Ca2+ signal
Leukotriene B4
Mast cell
Mitogen-activated protein kinase
Substance P
NDC
Medical sciences
abstract
Substance P (SP) selectively activates mast cells that reside in connective tissues. We studied the reactions of bone marrow-derived mast cells (BMMC) of three mouse strains, cultured with or without fibroblasts. BMMC co-cultured with fibroblasts, but not those cultured alone, increased intracellular Ca2+, released LTB4 and histamine in response to SP. PD098059 significantly inhibited the release of LTB4, but not histamine in all strains. SB203580 failed to reduce or slightly impaired the release of LTB4. These results suggest that mast cells undergo maturation under the influence of fibroblasts, acquiring the responsiveness to SP with Ca2+ signals and predominantly ERK-MAP kinase.
journal title
Journal of Neuroimmunology
volume
Volume 181
issue
Issue 1-2
start page
1
end page
12
date of issued
2006-12
publisher
Elsevier B.V.
issn
0165-5728
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Elsevier B.V.
relation is version of URL
http://dx.doi.org/10.1016/j.jneuroim.2006.07.011
department
Graduate School of Biomedical Science