Protein kinase Cδ binds TIRAP/Mal to participate in TLR signaling
Use this link to cite this item : https://ir.lib.hiroshima-u.ac.jp/00020576
ID | 20576 |
file | |
creator |
Kubo-Murai, Miho
Sukenobu, Naoe
Yoshikawa, Kyoko
Inoue, Kazumi
Yamamoto, Toshiyoshi
Matsumoto, Misako
Seya, Tsukasa
Inoue, Norimitsu
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subject | PKCδ
TLR
macrophage
signal transduction
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NDC |
Medical sciences
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abstract | Toll-like receptor (TLR) family members recognize specific molecular patterns within pathogens. Signaling through TLRs results in a proximal event that involves direct binding of adaptor proteins to the receptors. We observed that TIRAP/Mal, an adaptor protein for TLR2 and TLR4, binds protein kinase Cδ (PKCδ). TIRAP/Mal GST-fusion protein and a TIRAP/Mal antibody were able to precipitate PKCδ from rat peritoneal macrophage and THP1 cell lysates. Truncation mutants of TIRAP/Mal showed that the TIR domain of TIRAP/Mal is responsible for binding. TLR2- and TLR4-mediated phosphorylation of p38 MAPK, IKK, and IκB in RAW264.7 cells were abolished by depletion of PKCδ. These results suggest that PKCδ binding to TIRAP/Mal promotes TLR signaling events.
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journal title |
Molecular Immunology
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volume | Volume 44
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issue | Issue 9
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start page | 2257
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end page | 2264
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date of issued | 2007-03
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publisher | Pergamon-Elsevier Science Ltd.
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issn | 0161-5890
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ncid | |
publisher doi | |
pubmed id | |
language |
eng
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nii type |
Journal Article
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HU type |
Journal Articles
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DCMI type | text
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format | application/pdf
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text version | author
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rights | Copyright (c) 2006 Elsevier Ltd
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relation url | |
department |
Graduate School of Biomedical Science
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