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ID 20576
file
creator
Kubo-Murai, Miho
Sukenobu, Naoe
Yoshikawa, Kyoko
Inoue, Kazumi
Yamamoto, Toshiyoshi
Matsumoto, Misako
Seya, Tsukasa
Inoue, Norimitsu
subject
PKCδ
TLR
macrophage
signal transduction
NDC
Medical sciences
abstract
Toll-like receptor (TLR) family members recognize specific molecular patterns within pathogens. Signaling through TLRs results in a proximal event that involves direct binding of adaptor proteins to the receptors. We observed that TIRAP/Mal, an adaptor protein for TLR2 and TLR4, binds protein kinase Cδ (PKCδ). TIRAP/Mal GST-fusion protein and a TIRAP/Mal antibody were able to precipitate PKCδ from rat peritoneal macrophage and THP1 cell lysates. Truncation mutants of TIRAP/Mal showed that the TIR domain of TIRAP/Mal is responsible for binding. TLR2- and TLR4-mediated phosphorylation of p38 MAPK, IKK, and IκB in RAW264.7 cells were abolished by depletion of PKCδ. These results suggest that PKCδ binding to TIRAP/Mal promotes TLR signaling events.
journal title
Molecular Immunology
volume
Volume 44
issue
Issue 9
start page
2257
end page
2264
date of issued
2007-03
publisher
Pergamon-Elsevier Science Ltd.
issn
0161-5890
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Elsevier Ltd
relation url
department
Graduate School of Biomedical Science