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ID 25892
file
creator
Suprapto, Hari
Hara, Takaaki
Muroga, Kiyokuni
subject
Edwardsiella tarda
toxin
extracellular product
intracellular component
Japanese eel
ECP
NDC
Fishing industry. Fisheries
abstract
A toxin lethal to fish was purified from extracellular products (ECP) of Edwardsiella tarda by ion exchange chromatography and gel filtration. The purified toxin is a protein having proteolytic activities and lethal toxicity to Japanese eel Anguilla japonica with the LD50 of 1.6µg per g of fish body weight. Molecular weight of the protein was estimated to be 37 kDa by SDS-PAGE. SDS-PAGE, Western blot and imrnunodiffusion analyses revealed that the 37 kDa protein was present in the ECP and ICC (intracellular components) of virulent strains of E. tardy but not in those of avirulent strains, indicating that this toxin plays an important role in the pathogenicity of E. tarda.
journal title
Fish Pathology
volume
Volume 31
issue
Issue 4
start page
203
end page
207
date of issued
1996-12
publisher
日本魚病学会
issn
0388-788X
ncid
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biosphere Science