このエントリーをはてなブックマークに追加
ID 25891
file
creator
Fukuda, Yutaka
Nguyen, Huu Dung
Furuhashi, Makoto
subject
sevenband grouper
Epinephelus septemfasciatus
viral nervous necrosis
VNN
nodavirus
NDC
Fishing industry. Fisheries
abstract
Mass mortalities of sevenband grouper Epinephelus septemfasciatus reared in Oita Prefecture were investigated. The outbreaks of the disease occurred in summer seasons (July to October; water temperature 25-28°C) of 1993 and 1994. Affected fish ranging 170 to 1,850g in body weight were characterized by upside down swimming and inflation of swimbladder. Histopathologically, necrosis and vacuolation in the central nervous tissues and retina were observed. Although bacteria or parasites associated with the disease were not detected from affected fish and no CPE was observed on RTG-2, BF-2, and EPC cell lines, spherical virus particles, 28 nm in diameter, were found abundantly in the cytoplasm of affected nerve cells of most of the affected fish. The virus was identified as a fish nodavirus (nervous necrosis virus), based on the results of fluorescent antibody technique test with anti-SJNNV rabbit serum and polynnerase chain reaction test with primers for amplification of SJNNV coat protein gene. These results suggest that the mortalities of sevenband grouper were at least partly due to viral nervous necrosis (VNN). This is the first report describing VNN of sevenband grouper and the first case of VNN in the adult fish.
journal title
Fish Pathology
volume
Volume 31
issue
Issue 3
start page
165
end page
170
date of issued
1996-09
publisher
日本魚病学会
issn
0388-788X
ncid
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biosphere Science