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ID 25888
file
creator
Nishizawa, Toyohiko
Kise, Masaaki
Muroga, Kiyokuni
subject
monoclonal antibody
nodavirus
SJNNV
neutralization
striped jack
viral nervous necrosis
VNN
NDC
Fishing industry. Fisheries
abstract
Viral nervous necrosis (VNN) caused by a nodavirus (striped jack nervous necrosis virus: SJNNV) is a serious problem in seed production process of striped jack Pseudocaranx dentex in Japan. Mouse monoclonal antibodies (MAbs) to SJNNV were produced by using a homogenate of infected larval striped jack. Among 8 MAbs reacting with the homogenate of infected larvae in enzyme-linked immunosorbent assay (ELISA), three MAbs (SJ-102B, SJ-204D, SJ-207C) were proved to recognize the 42 kDa coat protein of SJNNV by Western blot analysis. An in vivo neutralization test, where larval striped jack were exposed to purified SJNNV (100 ng/ml) or the virus treated with MAbs (1 mg) and the resultant infection with SJNNV in larvae was monitored by ELISA using an anti-SJNNV rabbit serum, exhibited that two MAbs SJ-102B and SJ-204D have neutralizing activity against SJNNV.
journal title
Fish Pathology
volume
Volume 30
issue
Issue 2
start page
111
end page
114
date of issued
1995-06
publisher
日本魚病学会
issn
0388-788X
ncid
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biosphere Science