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ID 25671
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title alternative
Edwardsiella tarda のHEp-2細胞への付着性に及ぼす培地食塩濃度の影響
creator
Mahmoud Mostafa Mahmoud
Okuda, Jun
subject
Edwardsiella tarda
adherence
hemagglutination
HEp-2 cells
N-acetylneuraminic acid
NDC
Zoology
abstract
異なる食塩濃度の培地で培養したE. tarda 3株のHEp-2細胞への付着性を比較した。生菌数測定, 直接光顕観察のいずれにおいても, FK1051株およびKG8401株は3%食塩濃度の培地で培養すると0%食塩濃度の培地での培養に比べて高い細胞付着性を示したが, SU166株のそれは培地の塩分濃度にかかわらず低かった。これらの細胞付着性と培地食塩濃度との関係は, 赤血球凝集活性とのそれとよく一致した。また, この細胞付着性はN-アセチルノイラミン酸によって阻害された。
abstract
The adherence of Edwardsiella tarda strains to HEp-2 cells was studied by the viable cell count and direct microscopic count methods. Three selected E. tarda strains having different hemagglutination activities against guinea pig erythrocytes were cultured in a peptone-yeast extract broth supplemented with 3% NaCl (3%-NaCl culture) or without NaCl (0%-NaCl culture). Strain FK1051 showed significantly higher adherence in the 3%-NaCl culture than in the 0%-NaCl culture. A similar adherence pattern was observed in strain KG8401, though the adherence of the 0%-NaCl culture was higher than that of FK1051. In contrast, both cultures of strain SU166 were low in the adherence. These adherence patterns to the HEp-2 cells correlated well with the previously reported hemagglutinating activities of the strains. N-acetylneuraminic acid markedly inhibited the adherence as well as the hemagglutination. This NaCl-enhanced adherence may be involved in pathogenesis of E. tarda infection particularly in marine fish.
journal title
Fish Pathology
volume
Volume 41
issue
Issue 4
start page
165
end page
170
date of issued
2006-12
publisher
日本魚病学会
issn
0388-788X
1881-7335
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) 2007 The Japanese Society of Fish Pathology
relation url
department
Graduate School of Biosphere Science