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ID 25670
file
title alternative
Vibrio anguillarum のキノロン耐性機構
creator
Okuda, Jun
Kanamaru, Shunsuke
Yuasa, Akihiko
Nakaoka, Noriyoshi
Kawakami, Hidemasa
subject
Vibrio anguillarum
quinolone resistance
gyrA
parC
oxolinic acid
NDC
Zoology
abstract
Vibrio anguillarum のキノロン耐性機構を解明するために, DNAジャイレース遺伝子 (gyrA ) およびトポイソメラーゼIV遺伝子 (parC ) のキノロン耐性決定領域 (QRDR) 内の変異を解析した。低度耐性株ではgyrA のQRDRにのみ1塩基置換が検出された。一方, 高度耐性株ではgyrA とparC の両方のQRDRに1塩基置換が検出された。他菌種におけるキノロン耐性と同様, V. anguillarum においてもgyrA がまず変異することで低度耐性化し, 次にparC にも変異が入ることで高度耐性化すると考えられる。
abstract
To study a possible quinolone resistance mechanism in Vibrio anguillarum, we examined gyrA and parC mutations among the oxolinic acid-resistant strains and laboratory-derived mutants. One point mutation only in the quinolone resistance-determining region (QRDR) of the gyrA gene was detected in one strain isolated from ayu Plecoglossus altivelis and laboratory-derived mutants with low-level resistance. On the other hand, point mutations in both the gyrA and parC QRDRs were detected in one strain isolated from ayu and a laboratory-derived mutant with high-level resistance. These results suggest that as in other Gram-negative bacteria GyrA and ParC are the primary and secondary targets, respectively, of quinolone in V. anguillarum.
journal title
Fish Pathology
volume
Volume 41
issue
Issue 2
start page
73
end page
75
date of issued
2006-06
publisher
日本魚病学会
issn
0388-788X
1881-7335
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) 2007 The Japanese Society of Fish Pathology
relation url
department
Graduate School of Biosphere Science