このエントリーをはてなブックマークに追加
ID 28687
file
title alternative
Induction of Antibody Producing Cells in Some Fishes by Immunization with Vibrio anguillarum O-antigen
creator
Nakamura, Yoshinao
Muroga, Kiyokuni
NDC
Zoology
abstract
Vibrio anguillarum O抗原を腹腔内接種したコイ, ニジマス, アユ, ウナギ, ヒラメおよびマダニにおける抗体産生細胞の出現状況を, プラーク形成細胞(PFC)数を測定することにより調べた。PFC数は免疫8~17日後に最高値に達し, コイ, ニジマス, アユおよびウナギにおいては脾臓のPFC数が腎臓のPFC数より常に高かった。マダイでは脾臓と腎臓のPFC数はそれらとは逆になり, ヒラメでは差はなかった。O抗原で浸漬免疫したアユにおいては, 抗体産生細胞および抗体のいずれも検出されなかった。
abstract
Changes in the number of antibody producing cells were studied by measuring plaqueforming cells (PFC) of the spleen and kidney in carp (Cyprinus carpio), rainbow trout (Oncorhynchus mykiss), ayu (Plecoglossus altivelis), Japanese eel (Anguilla japonica), Japanese flounder (Paralichthys olivaceus), and red seabream (Pagrus major) after an intraperitoneal injection with Vibrio anguillarum O-antigen. It was confirmed in carp and ayu that the injected antigen was distributed at the highest levels in the spleen and kidney 3 to 6 h after the injection. PFCs appeared in the spleen and kidney of all the fish species tested. The number of PFC reached maximum value after 8-17 days. The number of PFC in the spleen was always higher than that in the kidney in carp, rainbow trout, ayu and eel, whereas in red seabream the former was lower than the latter. There was no difference in PFC number between the two organs in the Japanese flounder.

In an additional experiment, ayu was immunized by immersing in the O-antigen suspension (3 g/l) for 30 min. As a result, no PFCs were detected in the spleen or kidney and no aggulutinating antibody was measured in the serum.
journal title
魚病研究
volume
Volume 25
issue
Issue 4
start page
225
end page
230
date of issued
1990-12
publisher
日本魚病学会
issn
0388-788X
1881-7335
ncid
language
jpn
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) 1990 The Japanese Society of Fish Pathology
department
Graduate School of Biosphere Science