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ID 34737
file
creator
Ajiraporn, Kongpol
Alisa, S.Vangnai
subject
organic-solvent tolerant bacteria
organic-solvent tolerant mechanisms
cell adaptation
Exiguobacterium
NDC
Biology
abstract
A Gram-positive marine bacterium, Exiguobacterium sp. SBH81, was isolated as a hydrophilic organic-solvent tolerant bacterium, and exhibited high tolerance to various types of toxic hydrophilic organic solvents, including acetonitrile, at relatively high concentrations (up to 6% [v/v]) under the growing conditions. Investigation of its tolerance mechanisms illustrated that it does not rely on solvent inactivation processes or modification of cell surface characteristics, but rather, increase of the cell size lowers solvent partitioning into cells and the extrusion of solvents through the efflux system. A test using efflux pump inhibitors suggested that secondary transporters, i.e. resistance nodulation cell division (RND) and the multidrug and toxic compound extrusion (MATE) family, are involved in acetonitrile tolerance in this strain. In addition, its acetonitrile tolerance ability could be stably and significantly enhanced by repetitive growth in the presence of toxic acetonitrile. The marked acetonitrile tolerance of Exiguobacterium sp. SBH81 indicates its potential use as a host for biotechnological fermentation processes as well as bioremediation.
journal title
Microbes and Environments
volume
Volume 27
issue
Issue 1
start page
30
end page
35
date of issued
2012
publisher
Japanese Society of Microbial Ecology, Department of Bioresource Science
issn
1342-6311
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
(c) 2012 the Japanese Society of Microbial Ecology (JSME) / the Japanese Society of Soil Microbiology (JSSM). All Rights are Reserved.
relation url
department
Graduate School of Advanced Sciences of Matter