このエントリーをはてなブックマークに追加
ID 21548
file
creator
Tsutsumi, Shinichi
Miyazaki, Kazuko
Pan, Haiou
Koike, Chika
Yoshida, Eri
Takagishi, Kenji
Kato, Yukio
subject
mesenchymal stem cell
FGF
multilineage differentiation potential
life span
NDC
Medical sciences
abstract
Mesenchymal stem cells (MSC) that can differentiate to various connective tissue cells may be useful for autologous cell transplantation to defects of bone, cartilage, and tendon, if MSC can be expanded in vitro. However, a short life span of MSC and a reduction in their differentiation potential in culture have limited their clinical application. The purpose of this study is to identify a growth factor(s) involved in self-renewal of MSC and the maintenance of their multilineage differentiation potential. Fibroblast growth factor-2 (FGF-2) markedly increased the growth rate and the life span of rabbit, canine, and human bone marrow MSC in monolayer cultures. This effect of FGF-2 was more prominent in low-density cultures than in high-density cultures. In addition, all MSC expanded in vitro with FGF-2, but not without FGF-2, differentiated to chondrocytes in pellet cultures. The FGF(+) MSC also retained the osteogenic and adipogenic potential throughout many mitotic divisions. These findings suggest that FGFs play a crucial role in self-renewal of MSC.
journal title
Biochemical and Biophysical Research Communications
volume
Volume 288
issue
Issue 2
start page
413
end page
419
date of issued
2001-10-26
publisher
Elsevier
issn
0006-291X
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2001 Academic Press
relation url
department
Graduate School of Biomedical Science