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ID 17486
file
title alternative
活性酸素種曝露による血管内皮細胞単層膜のアルブミン透過性 : 細胞膜カルシウムチャネルの可逆的な機能変化
creator
subject
Albumin permeability
Reactive oxygen intermediates
Calcium channels
Redox state
NDC
Medical sciences
abstract
This study was designed to test the idea that the redox state of sulfhydryl (SH)-groups in cell-membrane Ca2+ channels plays a pivotal role in Ca2+in flux, which in turn causes an increase in albumin permeability across the cultured monolayer of porcine pulmonary artery endothelial (PPAE) cells exposed to xanthine/xanthine oxidase (X/XO). Albumin permeability as well as the concentration of intracellular Ca2+( [Ca2+]i) was increased by XKO. A Hz02 scavenger (catalase), an iron chelator (o-phenanthroline), and a hydroxyl radical scavenger (dimethyl sulfoxide) inhibited these changes provoked by XKO, in which intracellular iron-catalyzed hydroxyl radical generation was suggested to be involved. The increase in albumin permeability and [Ca2+]i continued once the PPAE cells were exposed to XKO. The [Ca2+]wi as decreased by a Ca2+ channel blocker, Ni2+, while the removal of Ni2+in creased [Ca2+]I again, suggesting the sustained Ca2+ influx through cell-membrane Ca" channels was responsible for the [Ca2+]i elevation. Ni2+f ailed to inhibit albumin permeability sustained after the removal of XKO. In contrast, SH-reducing agents (dithiothreitol and glutathione) inhibited the sustained permeability as well as Ca2+ influx. We concluded that the redox alteration of SH-groups in cell-membrane Ca2+ channels was involved in the increase in albumin permeability after exposure of the endothelial cells to oxidative stress.
description
Hiroshima J. Med. Sci. Vol.49, No.1, 57-65, March, 2000 HIJM49-8
language
eng
nii type
Thesis or Dissertation
HU type
Doctoral Theses
DCMI type
text
format
application/pdf
rights
Copyright (c) by Author
grantid
甲第2110号
degreeGrantor
広島大学(Hiroshima University)
degreename Ja
博士(医学)
degreename En
Medicine
degreelevel
doctoral
date of granted
2000-03-07
department
Graduate School of Biomedical Science