このエントリーをはてなブックマークに追加
ID 25934
file
creator
subject
CFTR
ENaC
Fluid accumulation
Mouse uterus
SERM
NDC
Medical sciences
abstract
Objectives: Estrogen regulates the expression of epithelial Na+ channel (ENaC) and cystic fibrosis transmembrane conductance regulator (CFTR). Our purpose was to assess the effects of raloxifene analogue LY117018 on the expression of ENaC and CFTR in ovariectomized mice.

Study design: Three groups of 5 female ovariectomized mice were treated with 17β-estradiol benzoate (E2), LY117018 (LY), or vehicle, respectively, for 4 to 12 weeks. Effects on the mRNA expression levels of ENaC and CFTR channels in the uterus were studied using real-time reverse transcriptase-polymerase chain reaction.

Results: E2 treatment induced CFTR expression, repressed ENaC expression and resulted in fluid accumulation in the uterus. In contrast, LY induced CFTR expression, did not repress ENaC expression, and caused no fluid accumulation.

Conclusions: Estradiol and LY117018 differentially regulate the expression of CFTR and ENaC in ovariectomized mouse uterus. This finding suggests that uterine fluid accumulation can be controlled mainly by targeting the ENaC.
journal title
American Journal of Obstetrics & Gynecology
volume
Volume 199
issue
Issue 4
start page
363.e1
end page
363.e6
date of issued
2008-10
publisher
Mosby
Elsevier
issn
0002-9378
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2008 Mosby, Inc.
relation url
department
Graduate School of Biomedical Science
Hiroshima Synchrotron Radiation Center