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ID 20760
file
creator
Taniguchi, Koji
Nomura, Kazutaka
Hata, Yumehiro
Nishimura, Tomoki
Asami, Yasuo
subject
silicon device
protein targeting
silica
ribosomal protein L2
protein array
protein orientation
green fluorescent protein
luciferase
NDC
Chemistry
abstract
Targeting functional proteins to specific sites on a silicon device is essential for the development of new biosensors and supramolecular assemblies. Using intracellular lysates of several bacterial strains, we found that ribosomal protein L2 binds tightly to silicon particles, which have surfaces that are oxidized to silica. A fusion of E. coli L2 and green fluorescence protein adsorbed to the silica particles with a Kd of 0.7 nM at pH 7.5 and also adsorbed to glass slides. This fusion protein was retained on the glass slide even after washing for 24 h with a buffer containing 1 M NaCl. We mapped the silica-binding domains of E. coli L2 to amino acids 1-60 and 203-273. These two regions seemed to cooperatively mediate the strong silica-binding characteristics of L2. A fusion of L2 and firefly luciferase also adsorbed on the glass slide. This L2 silica-binding tag, which we call the "Si-tag", can be used for one-step targeting of functional proteins on silica surfaces.
journal title
Biotechnology and Bioengineering
volume
Volume 96
issue
Issue 6
start page
1023
end page
1029
date of issued
2007
publisher
John Wiley & Sons, Inc.
issn
0006-3592
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Wiley Periodicals, Inc.
relation url
department
Graduate School of Advanced Sciences of Matter