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ID 14650
file
creator
Na, Kyung-su
Takiguchi, Noboru
Ikeda, Tsukasa
Ohtake, Hisao
Kato, Junichi
subject
solvent-tolerant bacterium
rhodococcus opacus
benzene-oxidizing bacterium
two-phase system
benzene dioxygenase gene mutation
NDC
Biology
abstract
Twenty-two benzene-utilizing bacteria were isolated from soil samples. Among them, three isolates were highly tolerant to benzene. They grew on benzene when liquid benzene was added to the basal salt medium at 10–90% (v/v). Taxonomical analysis identified the benzene-tolerant isolates as Rhodococcus opacus. One of the benzene-tolerant isolates, designated B-4, could utilize many aromatic and aliphatic hydrocarbons including benzene, toluene, styrene, xylene, ethylbenzene, propylbenzene, n-octane and n-decane as sole sources of carbon and energy. Strain B-4 grew well in the presence of 10% (v/v) organic solvents that it was capable of using as growth substrates. Genetic analysis revealed the benzene dioxygenase pathway is involved in benzene catabolism in strain B-4. A deletion-insertion mutant defective in the benzene dioxygenase large and small subunits genes (bnzA1 and bnzA2) was as tolerant to organic solvents as the wild-type strain B-4, suggesting that utilization or degradation of organic solvents is not essential for the organic solvent tolerance of R. opacus B-4.
journal title
Journal of Bioscience and Bioengineering
volume
Volume 99
issue
Issue 4
start page
378
end page
382
date of issued
2005-01
publisher
日本生物工学会
issn
1389-1723
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2005 The Society for Biotechnology, Japan
relation is version of URL
http://dx.doi.org/10.1263/jbb.99.378
department
Graduate School of Advanced Sciences of Matter