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ID 14200
file
creator
Hong, Chang Soo
Shitashiro, Maiko
Ikeda, Tsukasa
Takiguchi, Noboru
Ohtake, Hisao
Kato, Junichi
subject
Aerotaxis
Pseudomonas aeruginosa
Chemotaxis
Behavioral response
Transducer
NDC
Biology
abstract
It was previously shown that the chemotaxis gene cluster 1 (cheYZABW) was required for chemotaxis. In this study, the involvement of the same cluster in aerotaxis is described and two transducer genes for aerotaxis are identified. Aerotaxis assays of a number of deletion–insertion mutants of Pseudomonas aeruginosa PAO1 revealed that the chemotaxis gene cluster 1 and cheR are required for aerotaxis. Mutant strains which contained deletions in the methyl-accepting chemotaxis protein-like genes tlpC and tlpG showed decreased aerotaxis. A double mutant deficient in tlpC and tlpG was negative for aerotaxis. TlpC has 45 0x1.e0b6p-891mino acid identity with the Escherichia coli aerotactic transducer Aer. The TlpG protein has a predicted C-terminal segment with 89 0dentity to the highly conserved domain of the E. coli serine chemoreceptor Tsr. A hydropathy plot of TlpG indicated that hydrophobic membrane-spanning regions are missing in TlpG. A PAS motif was found in the N-terminal domains of TlpC and TlpG. On this basis, the tlpC and tlpG genes were renamed aer and aer-2, respectively. No significant homology other than the PAS motif was detected in the N-terminal domains between Aer and Aer-2.
journal title
FEMS microbiology letters
volume
Volume 231
issue
Issue 2
start page
247
end page
252
date of issued
2004-02
publisher
Elsevier Science B.V.
issn
0378-1097
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2004 Federation of European Microbiological Societies.
relation is version of URL
http://dx.doi.org/10.1016/S0378-1097(04)00009-6
department
Graduate School of Advanced Sciences of Matter