Chemotaxis proteins and transducers for aerotaxis in Pseudomonas aeruginosa
Use this link to cite this item : https://ir.lib.hiroshima-u.ac.jp/00014200
ID | 14200 |
file | |
creator |
Hong, Chang Soo
Shitashiro, Maiko
Ikeda, Tsukasa
Takiguchi, Noboru
Ohtake, Hisao
Kato, Junichi
|
subject | Aerotaxis
Pseudomonas aeruginosa
Chemotaxis
Behavioral response
Transducer
|
NDC |
Biology
|
abstract | It was previously shown that the chemotaxis gene cluster 1 (cheYZABW) was required for chemotaxis. In this study, the involvement of the same cluster in aerotaxis is described and two transducer genes for aerotaxis are identified. Aerotaxis assays of a number of deletion–insertion mutants of Pseudomonas aeruginosa PAO1 revealed that the chemotaxis gene cluster 1 and cheR are required for aerotaxis. Mutant strains which contained deletions in the methyl-accepting chemotaxis protein-like genes tlpC and tlpG showed decreased aerotaxis. A double mutant deficient in tlpC and tlpG was negative for aerotaxis. TlpC has 45 0x1.e0b6p-891mino acid identity with the Escherichia coli aerotactic transducer Aer. The TlpG protein has a predicted C-terminal segment with 89 0dentity to the highly conserved domain of the E. coli serine chemoreceptor Tsr. A hydropathy plot of TlpG indicated that hydrophobic membrane-spanning regions are missing in TlpG. A PAS motif was found in the N-terminal domains of TlpC and TlpG. On this basis, the tlpC and tlpG genes were renamed aer and aer-2, respectively. No significant homology other than the PAS motif was detected in the N-terminal domains between Aer and Aer-2.
|
journal title |
FEMS microbiology letters
|
volume | Volume 231
|
issue | Issue 2
|
start page | 247
|
end page | 252
|
date of issued | 2004-02
|
publisher | Elsevier Science B.V.
|
issn | 0378-1097
|
publisher doi | |
pubmed id | |
language |
eng
|
nii type |
Journal Article
|
HU type |
Journal Articles
|
DCMI type | text
|
format | application/pdf
|
text version | author
|
rights | Copyright (c) 2004 Federation of European Microbiological Societies.
|
relation is version of URL | http://dx.doi.org/10.1016/S0378-1097(04)00009-6
|
department |
Graduate School of Advanced Sciences of Matter
|