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ID 45393
file
creator
Kageyama, Tetsushi
Kuwada, Tomonori
Ohara, Kenichi
Nouno, Aya
subject
agglutination reaction
bacterial count
Flavobacterium psychrophilum
Plecoglossus altivelis altivelis
strain-dependent
NDC
Animal husbandry
abstract
Bacterial cold water disease (BCWD) in ayu (Plecoglossus altivelis altivelis) has a serious impact on aquaculture and fisheries. There is known to be a significant difference among ayu strains with regard to mortality caused by BCWD. In this study, the immune response of different ayu strains against Flavobacterium psychrophilum infection was observed. One strain was resistant to infection by F. psychrophilum, and the other was susceptible to infection by the same bacteria. The number of bacteria in the body was observed in each ayu strain, and the change in bacterial counts was similar. However, there was a significant difference in bacterial count in the spleen between the two strains on days 6, 9, 12 and 15 after exposure. To observe the immune response against F. psychrophilum, agglutination assay using serum was performed. An agglutination reaction in the resistant ayu strain was observed in 4 out of 6 ayu on day 6 after exposure, while no reactions in the susceptible ayu strain were observed in any sampled fish until day 12. However, some reactions in the susceptible ayu strain were observed in surviving ayu. These results indicate that there is a correlation between the presence of bacterial multiplication and agglutination reaction against F. psychrophilum.
journal title
Journal of Veterinary Medical Science
volume
Volume 75
issue
Issue 12
start page
1571
end page
1575
date of issued
2013-07-30
publisher
The Japanese Society of Veterinary Science
公益社団法人日本獣医学会
issn
1347-7439
0916-7250
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
publisher
rights
Copyright (c) 2013 The Japanese Society of Veterinary Science
relation url
department
Graduate School of Biosphere Science