Journal of the Faculty of Fisheries and Animal Husbandry, Hiroshima University Volume 5 Issue 1
1963-12-20 発行

Gas Liquid Chromatography による魚肉腐敗生産物中のアミン類の分離

Separation of lower aliphatic amines present in the spoilt fish meat by gas liquid chromatography
Sato, Yoshikazu
Ito, Keiji
Matsumoto, Fumio
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abstract
In this study, the authors attempted to separate the lower aliphatic amines and ammonia present in the spoilt fish meat by using of Gas-Liquid Chromatography (G. L. C.). However, the separation is difficult and unsuccessful unless the adopted partition liquid is suitable. So the liquid phase for available has been investigated, and determined the column temperature and the carrier gas flow rate. Then the separation of a standard mixture of aliphatic primary, secondary and tertiary amines were achieved reasonably, and the detection of amines content in a spoilt fishsoluble were
taken for a test of the method established above.

The results are as follows:

(1) As partition liquid for the separation of lower aliphatic amines, triethanolamine, cetylalcohol and glycerol were excellent than n-hexadecane, paraffin wax and triethylen glycol. (Text-fig. 1.)
(2) Especially the column connected with 1 m triethanolamine and 4 m cetylalcohol was more effective. In this case, the more suitable condition of procedure was the temperature of column 98°C and the flow rate of carrier gas 30 ml/min. (Text-fig. 2 & 3.)
(3) Although the separation of a standard mixture of aliphatic primary, secondary, tertiary amines and ammonia were achieved reasonably, it is imperfect to estimate them quantitatively because of the tailing of methylamine, dimethylamine and n-buthylamine. (Text-fig. 4.)
(4) By the detection of amines present in a spoilt fish-soluble were obtained remarkable ammonia, considerable trimethylamine and a little methylamine, ethylamine, diethylamine and trimethylamine or iso-buthylamine. (Text-fig.5-1~3.)
description
本研究は文部省科学試験研究費補助金"フイッシュソルプルの製造I"C関する研究"の一部として実施したものであることを附記する.