Journal of the Faculty of Fisheries and Animal Husbandry, Hiroshima University Volume 5 Issue 1
1963-12-20 発行

Location of sperms in the oviduct of the domestic fowl with special reference to storage of sperms in the vaginal gland

Fujii, Shunsaku
Tamura, Tatsudo
The distribution and location of sperms in the fertile oviduct of the domestic fowl was observed. For this purpose, section preparations were made from the oviduct at varying intervals of time after the last mating or artificial insemination. The results obtained are summarized as follows.

1. Sperms were distributed constantly in abundance and observed for a long time after insemination both in the chalaziferous portion and at the beginning of the vagina. The sperms were actually located in the grooves of the mucosal folds and the chalaziferous glands of the chalaziferous portion and in the lumina of the vaginal glands at the beginning of the vagina. The chalaziferous portion had been described by VAN ORIMMELEN as location of sperms, but the vaginal glands were found by the present author as such for the first time.
2. The vaginal glands were large simple tubular ones, about 1 cm long, situated in the lamina propria of the mucosal folds, far from the vagi nal orifice. They see med to be special glands characterized by the presence of cholesterin-ester-like lipid.
3. More sperms were stored in the vaginal glands for a longer time than in the chalaziferous portion. Sperms were found in the chalaziferous portion 2 or 3 weeks after insemination and in the vaginal gland on the 27th day after insemination, although it was not clear whether they were active or dead.
4. The perms stored in the crypts of the chalaziferous portion were agglutinated in the depths of the grooves, forming bundle-like clump , shortly after insemination. They became scattered with the lapse of time after insemination. The sperms stored in the vaginal glands formed packed masses, which were not disintegrated for a Long time.
5. The sperms forming masses had generally a normal structure, but those distributed independently had an abnormal one, lacking tail or acrosome.
6. The migration of sperms into the vaginal glands was voluntary. This was confirmed by using various experimental methods of insemination. In other words, the vaginal glands seemed to have some factor which favored the survival of sperms. From these results, it is concluded that the vaginal glands may act as a preliminary reservoir for sperms until the sperms reach the infundibulum, where they are finally stored.