Two-year old carp, Cyprinus carpio, were kept in an out-door pond between May of 1975 and January of 1976. Blood samples were collected at the end of every seasons by cardiac puncture with a heparinized cyringe. The seasonal variation of hematocrit value, plasma total lipid, and plasma sugar levels were measured.
The main 6 fractions were separated from the plasma protein by cellulose acetate electrophoresis. The localization of albumin and globulin fractions on the cellulose acetate electrophoretic diagram was estimated by the preliminary fractionation of the plasma protein by a 50% saturated ammonium sulfate solution. The cellulose acetate electrophoretic analysis showed that the plasma total albumin content was higher in the warm than the cold season.
The main albumin component in red carp has been identified as a kind of lipoprotein in a previous study. However now, in this study, the lipid-containing albumin was separated into 2 components in a disc electrophoresis. The amount of lipid-containing albumins was high in the warm season. The total protein content was also high in the same season. Therefore the fluctuation of carp plasma albumin seems to be connected to the seasonal factor. It was suggested that the variation of lipid-containing albumin had an influence on the plasma total protein level. However, no correlation was observed between the variations of the lipid-containing albumin and plasma total lipid.