The present experiment was undertaken in order to determine the diluents suitable for the freeze-drying and reconstitution of fowl spermatozoa. Whole milk, egg yolk citrate solution, gelatin, egg yolk glucose and glycerol egg yolk glucose solution were used for the extender of semen. The results are as follows;
1. In the semen samples diluted with glycerol egg yolk glucose solution, motile spermatozoa were observed at every experiment and the spermatozoa in some case showed 20 percent motility (over ++) and the percentage of motile spermatozoa recovered averaged 37 percent.
2. In the semen samples diluted with egg yolk glucose solution following freeze-drying and reconstitution, motile spermatozoa were not observed with the exception of one single experiment which the motility showed 10 percent (over ++) and the percentage of motile spermatozoa recovered averaged 10 percent.
3. In semen sample diluted with the other three diluents, i.e., whole milk, egg yolk citrate solution and gelatin, motile spermatozoa were not observed at all.
4. Therefore, out of above 5 diluents glycerol egg yolk glucose solution seemed to be the most adequate medium for withstanding freeze-drying preservation of fowl spermatozoa.