1. The blue and the purple carotenoproteins with absorption maxima at 655 and 615 mµ in 0.05 M phosphate buffer at pH 7.3 were obtained from the exoskeleton of the freshwater crayfish (Cambarus clarkii). Besides the above carotenoproteins, the red carotenoprotein which revealed a weak absorption around 475 mµ in visual region was separated in the exoskeleton.
2. Three kinds of carotenoproteins were distributed in various colored exoskeleton in different proportions. In the claws and limbs, the red carotenoprotein was found in somewhat larger degree than in the bluish carapace.
3. A fair amount of carotenoid-pigment remained in the exoskeleton after extraction by aqueous solution. This suggested the coexistence of carotenoproteins and unbound carotenoids in the pigmented exoskeleton.