The purpose of the present study was to create 3D images of living things using a 3D scanning applications, and to examine the effectiveness of the application as a tool for creating image materials in biology and practical use of the created 3D images as teaching materials. Three types of applications for iOS devices were used in the present study. As the result, in the case of the “QLONE”, delicate objects such as insect legs, tree branches or leaves were not scanned accurately. In the case of the “TRNIO”, insect legs were scanned accurately than the "QLONE". However, leaves and fine tree branches were not scanned. The appearance of the tree trunk was well scanned as the 3D images with the "3d Scanner App", and the epiphytic state of lichens could be recognized. When applying a 3D image as a teaching material for observation in a science class, the low resolution of the 3D images prevent students from recognizing the fine morphologies. Therfore, it is better not to observe only the 3D image but to morphologically compare it with the actual subject.