Hiroshima journal of school education Volume 22
2016-03-22 発行

簡易蛍光顕微鏡の開発と高等学校生物における活用の提案

Development of a simple, hand made fluorescence microscope and a proposal to its practical use for the high school biology
Saiga, Daisuke
Maga, Ayane
Kinutani, Kazuko
Fukuda, Mika
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abstract
In the present study, a simple hand made fluorescence microscope was developed. Four UV-LED lamps (NS360L-5RLO, Nitride Semiconductors Co., Ltd.) were used for the ultraviolet irradiation device. Some problems of a sample device have been improved and that has been completed. As the result, the nuclear fluorescence of the epidermal tissue of Tradescantia ohiensis stained by DAPI (4',6-diamidino-2-phenylindole) were observed.

This low cost simple fluorescence microscope was very effective for the student’s activities in the high school biology classes. In this paper, authors proposed an example of experiment which analyze relative DNA content value of nucleus in mitotic cell cycle by using this fluorescence microscope.
description
本研究の一部は,科学研究費基盤研究A(25242015)および科学研究費基盤研究C(26350235)の助成を受けて行ったものである。