The detergency of the mixture of hexadecyl trimethylammonium bromide (HTAB) and dodecyl polyoxyethylene ether (DPE) was studied using cotton and polyester fabric. The critical micell concentration of the mixture determined by surface tension method was 4×10-4 mol/dm3. Polystyrene latex particle removal and redeposition, removal of liquid paraffin and oleic acid, sterilization of staphylococcus aureus, deodorization of stench of cigarette and safety were examined through SEM observation, spectrophtometry, culture, a smell meter and a skin model. The effect of HTAB/DPE combination ratio on these six detergency indexes was investigated. The most effective mixing ratio of the mixture for detergency except oily and fatty soil removal was 90:10. When the combination ratio of HTAB was more than 90 %, the detergency reduced for reason of the increase of the surface tension of the mixture. The more DPE content was in the mixture, the more the oily and fatty soil was removed. The survival rate of the cells of the skin model was from 90 % to 100 % same as water. Therefore it was cleared that the mixture of HTAB and DPE used this study was safety.