Aquatic animals are often treated as teaching materials for the environmental studies. For the effective practices, the basic data of aquatic animals that inhabit to the target region is necessary. In the present study, the distribution of aquatic animals (fishes and aquatic insects) and the area of their habitats were investigated at the Kageura river and the Kumano river of the Seno river system (Hiroshima Pref.). As the results, 14 species of fishes and 40 species of aquatic insects were recognized. On the bases of these data, some teaching materials were shown. They were the atlas, the distribution map and the web contents.