Journal of the Graduate School of Biosphere Science, Hiroshima University Volume 54
2015-12-25 発行

瀬戸内海産ミズクラゲのポリプクローン系統の確立と特徴

Characterization of Clonal Polyp Strains Established from Aurelia sp. Inhabiting the Seto Inland Sea of Japan
Tsujita, Natsumi
Kuroda, Rie
Okumura, Izumi
Yoshioka, Sayaka
Nakatani, Minami
Koyama, Hiroki
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abstract
In the life cycle of Aurelia sp., the transition from polyp to strobila/ephyra is called“strobilation”. To study the molecular mechanisms of strobilation with molecular biological and biochemical approaches, we established 7 clonal polyp strains from Aurelia sp. inhabiting the Seto Inland Sea of Japan. In laboratory condition, the clonal strains started strobilation in 32 - 42 days by a temperature change from 25˚C to 10˚C, or in 4 - 8 days at 25˚C by administration of indomethacin (25 μM). The experiments of short-term low-temperature treatments revealed that strobilation proceeded to the end independently of temperature after strobilation had been initiated. Further, molecular phylogenetic analysis suggested that the clonal strains could be Aurelia sp. 1 (Dawson et al., 2005).
description
本研究は,平成20年度広島大学後援会・教育研究一般助成および平成20年度広島大学大学院生物圏科学研究科・研究科長裁量経費研究助成の支援のもとで実施されたものである。
subject
クローン系統
ストロビレーション
瀬戸内海
分子系統解析
ポリプ
ミズクラゲ
Aurelia sp.
clonal strain
molecular phylogenetic analysis
polyp
strobilation
the Seto Inland Sea