Stable isotopes can provide useful knowledge about sources and processes within an ecosystem. The stable isotopes of carbon (δ13C) and nitrogen (δ15N) were used to investigate trophic relationships of relatively commercially important 21 finfish species, cephalopods in Hiroshima Bay. Among 21 finfish species, the lowest mean δ15N of 14.4‰ was recorded for Engraulis japonicus and Hyporhamphus sajori while the highest mean δ15N of 16.8‰ was recorded for Sebasticus marmoratus. The lowest and highest mean δ13C were noted -17.6‰ for Chromis notata and H. sajori, and -15.3‰ for Pagrus major and Sillago japonica, respectively. Including with cephalopods, the highest mean δ13C and δ15N noted at -14.8‰ and 17.3‰ for Sepioteuthis lessoniana. Information of stable isotope variation and trophic level in aquatic species of Hiroshima Bay can be used for monitoring and managing sustainable fisheries.