A specimen of Shokihaze goby, Tridentiger barbatus, was collected from Yamaguchi Bay, western Seto Inland Sea, Japan in June 2012. This species has been evaluated as a near threatened species in Japan. This specimen was an adult female of 110.1mm TL and 89.4mm SL. The gonadosomatic index was 1.59%. Histologically the most developed stage of normal oocytes was the secondary yolk globule stage. The specimen had a lot of new postovulatory follicles in the ovarian lamella and a few ovulated ripe oocytes remained in the ovarian cavity. Therefore, these results indicate that spawning has occurred and this species is a multiple spawner. This is the first report of the occurrence and spawning of T. barbatus, based on a specimens from Yamaguchi Bay.