Journal of the Graduate School of Biosphere Science, Hiroshima University Volume 50
2011-12-24 発行

Estimation of geographical distribution limits between two subspecies of white-spotted charr, Salvelinus leucomaenis imbrius and S. l. pluvius, on the basis of RAPD analysis

RAPD分析に基くイワナ2亜種,ゴギとニッコウイワナの地理的分布境界の推定
Inoue, Takanori
Imabayashi, Hiromichi
file
abstract
We estimated the distribution limits of the 2 subspecies of the white-spotted charr, Salvelinus leucomaenis (‘Iwana’), S. l. pluvius (‘Nikkoiwana’) and S. l. imbrius (‘Gogi’) by examining the distribution of specific genetic types to Nikkoiwana or Gogi in the rivers flowing into the Sea of Japan on the basis of Random Amplified Polymorphic DNA (RAPD). A total of 16 DNA fragments was amplified. Seven to 14 bands were detected from an individual. There were no common bands only to the Nikkoiwana or Gogi. Fifteen and 9 haplotypes were recorded for the Nikkoiwana and Gogi, respectively. Among these, only 2 haplotypes were common to both subspecies. In the intermediate region where both the species were possible to be distributed, 24 haplotypes were detected, among which 9 and 5 types were Nikkoiwana- and Gogi-specific, respectively. Nikkoiwana-specific types were distributed in westernmost to the Hino River, Tottori Prefecture, whereas Gogi-specific types were distributed in easternmost to the Katsuta River, Tottori Prefecture. For the Hino River Basin, 17 haplotypes were detected, among which 7 and 3 types were Nikkoiwana- and Gogi-specific, respectively. In a cladogram, there were no large clades comprising only Nikkoiwana- or Gogi-specific haplotypes. These results suggest westward and eastward range expansions for the Nikkoiwana and Gogi, respectively, and the existence of Mt. Daisen Mountain Mass as a barrier to expansion of both subspecies.
subject
distribution
Gogi
Nikkoiwana
RAPD
Salvelinus
分布
ゴギ
ニッコウイワナ
RAPD
イワナ属