We surveyed fish fauna at shallow waters of Aki Nada, Seto Inland Sea by the line fishing census, using small hooks attaching clamworms or krills as baits, during May 2007 - March 2008. We set up survey points at piers with Sargassum belt in Osaki-Kami Shima Island, and conducted the census (ca. 10 person x 60 min at each) bimonthly to analyze seasonal differences of fish fauna. In order to evaluate geographic variation of fish fauna, we also held the census at 26 survey points of nine zones situated a wide area in Aki Nada during May-July, 2007. A total of 63 species of 29 families were recorded. Of 63 species, 76 % were commonly recorded in Iyo Nada region. In contrast, the common species ratio fell to ca. 30% in comparison with data recorded in Uwa Sea region, which strongly supports the validity of the border of biogeographical regions between “Warm temperate region” including Uwa Sea and “intermediate temperate region” including Iyo Nada and Aki Nada (Nishimura, 1981). The almost year-round occurrences in the Osaki-Kami Shima were admitted in the following 18 species, Sebastiscus marmoratus, Sebastes inermis, Hypodytes rubripinnis, Hexagrammos agrammus, Pseudoblennius cottoides, Pagrus major, Ditrema temmincki, Chromis notata notata, Girella punctata, Semicossyphus reticulatus, Pseudolabrus sieboldi, Halichoeres poecilopterus, Halichoeres tenuispinnus, Parapercis sexfasciata, Istigobius hoshinonis, Cryptocentrus filifer, Takifugu pardalis, Takifugu poecilonotus. Most of these species commonly occurred in the survey zones in Aki Nada waters, suggesting that these are core components of fish fauna in shallow waters of Aki Nada.