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ID 50001
file
creator
MYOGA, Hiroshi
subject
Phosphatase
Corpus luteum
Cerium
Microslicer
Ultracytochemistry
NDC
Medical sciences
abstract
Acid phospatase (AcPase), alkaline phosphatase (AlPase), 5'-nucleotidase (AMPase), Mg-activated adenosine triphospatase (ATPase), thiamine pyrophospatase (TPPase) and glucose-6-phospatase (G6Pase) were investigated in the human corpus luteum of early pregnancy using a cerium-based method in combination with a microslicer.
Ultrastructurally, cerium phosphate reaction product appears as a very fine electron dense precipitate. The AcPase reaction products were exclusively localized in the lysosomes and in two cisternae of the Golgi lamellae on the trans-side. The AlPase and AMPase reaction products were observed in the plasma membrane. The Mg-activated ATPase reaction products were found in the matrix of the mitochondria as well as in the plasma membrane. The TPPase reaction products were present in two cisternae of the Golgi lamellae on the trans-side. The G6Pase reaction products were found in the rough endoplasmic reticulum. This technique can be applied routinely for the demonstration of phosphatase activity because of its reproducibility and specificity when cerium is used as the capturing agent, and yields better results than the lead-based method. In the luteal cells, these several phosphatases might serve to maintain a constant metabolic activity in the early pregnancy.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 36
issue
Issue 4
start page
347
end page
355
date of issued
1987-12
publisher
Hiroshima University Medical Press
issn
0018-2052
ncid
pubmed id
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biomedical Science
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