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ID 42769
file
title alternative
Modification of Culturing Method for Saprolegnia sp. as the Observation Material in Biology
creator
Takao, Akira
subject
Saprolegnia
life cycle
culturing method
NDC
Education
abstract
Saprolegnia spp., common aquatic fungi, are known as one of the appropriate material to observe the reproductive process in its life cycle. The aim of the present study is to modify culturing method of Saprolegnia sp. We prepared culture media containing various concentration of the Cannabis extract solidified by agar. The hyphae of Saprolegnia sp. were cultured in sterilized water with small pieces of the agar media. As the result, many zoosporangia and archegonia were observed under the light microscope. In comparison with the ordinary culturing method using whole cotyledon of Cannabis, the colonies of Saprolegnia sp. obtained by this modified method were suitable to observe various stage of life cycle. The nutritional conditions in culturing are able to be controlled by this method. Therefore, it will be used as the practical method in the biology class.
description
本研究は,科学研究費基盤研究(A)(一般)(25242015)および科学研究費基盤研究(C)(一般)(26350235)の助成を受けて行った。
journal title
Hiroshima journal of school education
volume
Volume 23
start page
27
end page
30
date of issued
2017-03-22
publisher
広島大学大学院教育学研究科附属教育実践総合センター
issn
1341-111X
ncid
language
jpn
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Education
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