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ID 41293
file
creator
Yuri, Shota
subject
Anammox
Chloroflexi
MAR-FISH
phylogenetic analysis
substrate uptake pattern
NDC
Construction. Civil engineering
abstract
The coexistence of uncultured heterotrophic bacteria belonging to the phylum Chloroflexi has often been observed in anaerobic ammonium oxidation (anammox) reactors fed with synthetic nutrient medium without organic carbon compounds. To determine if coexisting Chloroflexi in anammox reactors scavenge organic matter derived from anammox bacterial cells, the present study was conducted to investigate the substrate uptake pattern of the uncultured Chloroflexi present in an anammox reactor and to clarify if they take up microbial products derived from anammox bacterial cells. To accomplish this, combined microautoradiography and fluorescence in situ hybridization (MAR-FISH) was conducted. Phylogenetic analysis revealed that 36% of the clones analyzed in this study were affiliated with Chloroflexi. The sequence similarities to Anaerolinea thermophila and Caldilinea aerophila within the phylum Chloroflexi were only 81.0-88.7% and 80.3-83.8%, respectively. The uncultured Chloroflexi were found to incorporate sucrose, glucose, and N-acetyl-glucosamine. The 14C-tracing experiment revealed that the uncultured Chloroflexi were clearly MAR-positive, indicating the utilization of decaying anammox bacterial cell materials. Taken together, these results indicate that coexisting uncultured Chloroflexi in anammox reactors scavenge organic compounds derived from anammox bacterial cells.
description
This research was partially supported by an Assistance for Research and Development of Construction Technology grant from the Ministry of Land, Infrastructure, Transport and Tourism, and by a Grant-in-Aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology.
journal title
Water Science and Technology
volume
Volume 66
issue
Issue 12
start page
2556
end page
2561
date of issued
2012-10
publisher
IWA Publishing
issn
0273-1223
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) IWA Publishing 2012
relation is version of URL
http://doi.org/10.2166/wst.2012.479
department
Graduate School of Engineering



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