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ID 34981
file
creator
Tachibana, Noriko
Iwamoto, Takaaki
Kawamura, Toshiyuki
Masuda, Yuji
Mori, Toshio
subject
Translesion DNA synthesis
REV1
Antibody
NDC
Medical sciences
abstract
Continuous exposure of cells to exogenous and endogenous agents produces many types of DNA damage during normal cell cycles. Post-replication repair, consisting of error-free and error-prone sub-pathways, is required for tolerance of such DNA damage. REV1 plays a crucial role in regulation of the error-prone pathway. To facilitate analysis of its cellular functions, we here generated a mouse REV1 monoclonal antibody, called D6, which also recognizes human REV1. The epitope for the antibody could be mapped between 860-877 amino acid residues of human REV1, which was located outside of the conserved catalytic domain. Although the antibody unfortunately could not specifically detect endogenous mouse and human REV1 by western blotting and immunohistochemistry, we established a method to identify endogenous human REV1 by immunoprecipitation-western blotting analysis combining D6 and separately generated polyclonal antibodies.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 59
issue
Issue 3
start page
51
end page
56
date of issued
2010-09
publisher
Hiroshima University Medical Press
issn
0018-2052
ncid
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
rights
(c) Hiroshima University Medical Press.
department
Graduate School of Biomedical Science
Research Institute for Radiation Biology and Medicine
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