Characterization of Acetonitrile-Tolerant Marine Bacterium Exiguobacterium sp SBH81 and Its Tolerance Mechanism
Use this link to cite this item : http://ir.lib.hiroshima-u.ac.jp/00034737
ID | 34737 |
file | |
creator |
Ajiraporn, Kongpol
Alisa, S.Vangnai
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subject | organic-solvent tolerant bacteria
organic-solvent tolerant mechanisms
cell adaptation
Exiguobacterium
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NDC |
Biology
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abstract | A Gram-positive marine bacterium, Exiguobacterium sp. SBH81, was isolated as a hydrophilic organic-solvent tolerant bacterium, and exhibited high tolerance to various types of toxic hydrophilic organic solvents, including acetonitrile, at relatively high concentrations (up to 6% [v/v]) under the growing conditions. Investigation of its tolerance mechanisms illustrated that it does not rely on solvent inactivation processes or modification of cell surface characteristics, but rather, increase of the cell size lowers solvent partitioning into cells and the extrusion of solvents through the efflux system. A test using efflux pump inhibitors suggested that secondary transporters, i.e. resistance nodulation cell division (RND) and the multidrug and toxic compound extrusion (MATE) family, are involved in acetonitrile tolerance in this strain. In addition, its acetonitrile tolerance ability could be stably and significantly enhanced by repetitive growth in the presence of toxic acetonitrile. The marked acetonitrile tolerance of Exiguobacterium sp. SBH81 indicates its potential use as a host for biotechnological fermentation processes as well as bioremediation.
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journal title |
Microbes and Environments
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volume | Volume 27
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issue | Issue 1
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start page | 30
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end page | 35
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date of issued | 2012
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publisher | Japanese Society of Microbial Ecology, Department of Bioresource Science
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issn | 1342-6311
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ncid | |
publisher doi | |
language |
eng
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nii type |
Journal Article
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HU type |
Journal Articles
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DCMI type | text
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format | application/pdf
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text version | publisher
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rights | (c) 2012 the Japanese Society of Microbial Ecology (JSME) / the Japanese Society of Soil Microbiology (JSSM). All Rights are Reserved.
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relation url | |
department |
Graduate School of Advanced Sciences of Matter
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