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ID 31592
file
creator
Kominami, Yoko
Gon, Rina
Hayashi, Masaomi
Nishiki, Masayo
Sasaki, Atsushi
Shiraishi, Miho
Ueda, Shinobu
Ochiya, Takahiro
subject
Transplantation
Hetero-tissues
Duodenum
F344 GFP Rats
NDC
Medical sciences
abstract
The mechanisms regulating stem cell differentiation and self-renewal are largely unknown. Our ultimate goal is to be able to regulate somatic stem cell differentiation and proliferation, In the present study the ability of trans-differentiation was studied when different normal tissue types were transplanted into the duodenum in rats. Pieces of ear (skin), bladder, trachea, diaphragm, pyloric gland. and forestomach from 8-week old GFP-F344 rats were transplanted into the duodenum of F344 rats. Goblet cells with alcian-blue PAS positive mucin and brash border with alkaline phosphatase (ALP) activity appeared in tissues implanted into the duodenum. In addition, GFP-positive duodenal mucosa was observed in all cases by immunohistochemical staining. Moreover, the GFP-positive cells were found to carry the GFP transgene by PCR analysis. indicating that the bladder, trachea. ear (skin), diaphragm, pyloric gland, and farestomach tissues showed a multipotential ability for differentiation. These results indicated that stem cells within tissues have a multipotential ability, trans-differentiating into different organs when transplanted into different environments.
journal title
Hiroshima Journal of Medical Sciences
volume
Volume 60
issue
Issue 1
start page
1
end page
6
date of issued
2011-03
publisher
Hiroshima University Medical Press
issn
0018-2052
ncid
language
eng
nii type
Departmental Bulletin Paper
HU type
Departmental Bulletin Papers
DCMI type
text
format
application/pdf
text version
publisher
department
Graduate School of Biomedical Science
Research Institute for Radiation Biology and Medicine
Natural Science Center for Basic Research and Development
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