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ID 25930
file
creator
Makihira, Seicho
Kawahara, Yumi
Mine, Yuichi
subject
Osteoblast cells
Osteoclast cells
3D clinostat
Microgravity
RANKL
OPG
NDC
Medical sciences
abstract
Mechanical unloading conditions result in decreases in bone mineral density and quantity, which may be partly attributed to an imbalance in bone formation and resorption. To investigate the effect of mechanical unloading on osteoblast and osteoclast differentiation, and the expression of RANKL and OPG genes in osteoblasts, we used a three-dimensional (3D) clinostat system simulating microgravity to culture MC3T3-E1 and RAW264.7 cells. Long-term exposure (7 days) of MC3T3-E1 cells to microgravity in the 3D clinostat inhibited the expression of Runx2, Osterix, type I collagen αI chain, RANKL and OPG genes. Similarly, 3D clinostat exposure inhibited the enhancement of β3-integrin gene expression, which normally induced by sRANKL stimulation in RAW264.7 cells. These results, taken together, demonstrate that long-term 3D clinostat exposure inhibits the differentiation of MC3T3-E1 cells together with suppression of RANKL and OPG gene expression, as well as the RANKL-dependent cellular fusion of RAW264.7 cells, suggesting that long-term mechanical unloading suppresses bone formation and resorption.
journal title
Cell Biology International
volume
Volume 32
issue
Issue 9
start page
1176
end page
1181
date of issued
2008-09
publisher
Academic Press Inc.
Elsevier Science
issn
1065-6995
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2008 International Federation for Cell Biology Published by Elsevier Ltd.
relation url
department
Graduate School of Biomedical Science



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