このエントリーをはてなブックマークに追加
ID 25926
file
creator
Inoue, Kazumi
subject
Akt
Fcγreceptor
Phagocytosis
Phosphoinositide 3-kinase
PKCδ
NDC
Medical sciences
abstract
Stimulation of macrophages by various ligands results in the activation of both phosphoinositide 3-kinase (PI3K) and protein kinase C (PKC). Here, we showed that PKCδ selectively inhibits class IA PI3K. Prior exposure of macrophages to a PKC activator, phorbol 12-myristate 13-acetate (PMA) inhibited the PI3K activation induced by the Fcγ receptor (FcγR) ligation but not that induced by C5a. Prolonged PKC inhibition by GF109203X increased the basal PI3K activity of quiescent macrophages. The effect of the PKC inhibitor can be observed in macrophages from mice lacking class IB PI3K (p110γ). Thus PKC was suggested to selectively attenuate the class IA activity. Chronic PKC activation by PMA induced PKCδ degradation and Akt activation. Enhancement of the basal Akt actvity was also observed in cells stably deficient in PKCδ prepared by shRNA technique. FcγR-mediated phagocytosis was dramatically increased in these cells. Thus it is suggested that inactivation of class IA PI3K by PKCδ is functioning in regulation of FcγR-mediated phagocytosis.
journal title
The Journal of Biochemistry
volume
Volume 145
issue
Issue 1
start page
87
end page
94
date of issued
2009
publisher
Other Oxford University Press
issn
0021-924X
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2008 The Japanese Biochemical Society
relation url
department
Graduate School of Biomedical Science



Last 12 months's access : ? times
Last 12 months's DL: ? times


This month's access: ? times
This month's DL: ? times