このエントリーをはてなブックマークに追加
ID 20784
file
creator
Kawanishi, Michiko
Yoshida, Teruyoyo
Kijima, Mayumi
Yagyu, Kazuhiro
Okada, Sanae
Endo, Akihito
Murakami, Masaru
Morita, Hidetoshi
Suzuki, Shoko
subject
Broccoli sprout
Japan
Lactococcus garvieae
Phage and pulsed-field gel electrophoresis
Phathogenicity
Radish sprouts
Seriola
NDC
Industry and commerce
abstract
Aims: To identify Lactococcus garvieae isolates from radish and broccoli sprouts and compare them with virulent and less virulent mutant strains obtained from yellowtails with regard to KG phenotype, presence of a capsule and virulence towards yellowtails and mice. Methods and Results: Comparative 16S rRNA gene sequence analysis of six isolates obtained from radish and broccoli sprouts indicated that they were L. garvieae (similarity >99%). They were compared with KG9502, Lg2 and ATCC49156 strains obtained from yellowtails. A less virulent mutant strain Lg2-S was obtained by Lg2 subculture. Biochemical characterization of the six strains resembled that of KG9502, Lg2, ATCC49156 and Lg2-S, except for saccharose and tagatose acidification and the presence of hippuricase. These six strains were nonpathogenic towards yellowtails and mice, nonsusceptible to bacteriophages and demonstrated heterogeneity on pulsed-field gel electrophoresis analysis. Using transmission electron microscopy, a capsule was observed in KG9502 and Lg2 but not in ATCC49156 and Lg2-S. Conclusions: We isolated L. garvieae strains that lacked pathogenicity towards yellowtails and mice from radish and broccoli sprouts; these were noncapsulated and exhibited KG+ phenotype. Significance and Impact of the Study: This is the first documentation of L. garvieae isolated from terrestrial plants. These isolates exhibited genetic diversity; however, they were noncapsulated and nonpathogenic towards yellowtails and mice.
journal title
Letters in Applied Microbiology
volume
Volume 44
issue
Issue 5
start page
481
end page
487
date of issued
2007-05
publisher
Blackwell
issn
0266-8254
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2007 Authors,
relation
The definitive version is available at www.blackwell-synergy.com
relation is version of URL
http://dx.doi.org/10.1111/j.1472-765X.2007.02114.x
department
Graduate School of Biosphere Science



Last 12 months's access : ? times
Last 12 months's DL: ? times


This month's access: ? times
This month's DL: ? times