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ID 17245
file
creator
Fujiwara, Yukichi
Kozono, Keina
Iijima, Noriaki
Nagamatsu, Yasunori
Uematasu, Kazumasa
subject
c-fos
immedicate early gene
goldfish
kainic acid
NDC
Fishing industry. Fisheries
abstract
c-fos is an immediate early gene, and is rapidly and transiently induced in neurons of the central nervous system according to their activities. To investigate neuronal activities in the brain of the goldfish (Carassius auratus), we considered that expression of c-fos mRNA would be an available marker for the neuronal activities. Therefore, we firstly isolated a cDNA clone encoding c-Fos from the goldfish brain by RT-PCR and RACE methods. A full length cDNA of the goldfish c-fos was composed of 1,044 bp open reading frame. The amino acid sequence of the goldfish c-Fos was approximately 56-90 0dentical to those of teleost fish c-Fos. Northern blot analysis showed that the expression of c-fos mRNA was rapidly and transiently induced in the brain of the goldfish by the intraperitoneal administration of kainic acid. We also showed that the identification of the c-fos mRNA expression site by in situ hybridization will be able to use as an anatomical marker for identification of the activated neuronal region in the goldfish brain.
journal title
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
volume
Volume 1
issue
Issue 2
start page
253
end page
259
date of issued
2006-06
publisher
Elsevier Inc.
issn
1744-117X
ncid
publisher doi
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
relation is version of URL
http://dx.doi.org/10.1016/j.cbd.2005.12.005
department
Graduate School of Biosphere Science



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