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ID 17108
file
creator
Okuda, Jun
Arikawa, Yoko
Takeuchi, Yusuke
Mostafa, Mahmoud
Suzaki, Etsuko
Kataoka, Katsuko
Suzuki, Toshiharu
subject
Edwardsiella tarda
the type III secretion system
NF-кB
anti-apoptosis
intracellular replication
macrophage
NDC
Biology
abstract
Edwardsiella tarda is a pathogen with a broad host range that infects both animals and humans. Resistance to phagocytic killing may be involved in the pathogenicity of this bacterium. Here we show that intracellular replication of E. tarda in murine macrophages is dependent on the type III secretion system and induces an anti-apoptotic effect by up-regulating anti-apoptotic NF-κB target genes. The wild-type strain replicates within the phagosomal membrane of macrophages; whereas the type III mutant does not. Microarray analysis shows the mRNA expression level of NF-κB target genes (e.g. pro-inflammatory cytokines and anti-apoptotic genes) in macrophages infected with the wild-type strain were up-regulated compared to macrophages infected with the type III mutant. Up-regulation of Bcl2a1a, Bcl2a1b, cIAP-2, and TRAF1 genes induced expression of anti-apoptotic proteins to protect macrophages from apoptosis induced by staurosporine. Further, this protection was inhibited by adding kamebakaurin, an inhibitor of NF-κB activation and was confirmed using an NF-κB reporter gene assay. Up-regulation of anti-apoptotic NF-κB target genes is responsible for the anti-apoptotic activity of E. tarda and is required for intracellular replication in murine macrophages.
journal title
Microbial Pathogenesis
volume
Volume 41
issue
Issue 6
start page
226
end page
240
date of issued
2006-12
publisher
Elsevier
issn
0882-4010
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Elsevier Ltd.
relation is version of URL
http://dx.doi.org/10.1016/j.micpath.2006.08.002
department
Graduate School of Biosphere Science



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