このエントリーをはてなブックマークに追加
ID 17057
file
creator
Oku, Hiromi
Koizumi, Naoto
Okumura, Takuji
Kobayashi, Takanori
subject
teleost
red sea bream
Pagrus major
LPL
HL
PL
nutritional regulation
molecular cloning
NDC
Fishing industry. Fisheries
abstract
To investigate the nutritional regulation of lipid metabolism in fish, molecular characterization of lipases was conducted in red sea bream Pagrus major, and the effects of fasting and refeeding on their gene expression was examined. Taken together with our previous study (LPL2: Oku et al. 2002, Comp. Biochem. Physiol. 131B, 775-783), four lipase genes were identified and characterized as lipoprotein lipase (LPL), hepatic lipase (HL) and pancreatic lipase (PL). These four lipase genes, termed LPL1, LPL2, HL and PL, share a high degree of similarity with each other. Both LPL1 and LPL2 genes were expressed in various tissues including adipose tissue, gill, heart and hepatopancreas. HL gene was exclusively expressed in hepatopancreas. PL gene expression was detected in hepatopancreas and adipose tissue. Red sea bream LPL1 and LPL2 gene expression levels in hepatopancreas were increased during 48 hr fasting and decreased after refeeding, whereas no significant change in the expression levels of LPL1 and LPL2 was observed in adipose tissue, indicating that LPL1 and LPL2 gene expression is regulated in a tissue-specific manner in response to the nutritional state of fish. HL and PL gene expression was not affected by fasting and refeeding. The results of this study suggested that LPL, HL and PL gene expression is under different regulatory mechanisms in red sea bream with respect to the tissue-specificities and their nutritional regulation.
journal title
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
volume
Volume 145
issue
Issue 2
start page
168
end page
178
date of issued
2006-10
publisher
Elsevier
issn
1096-4959
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2006 Elsevier Ltd.
relation is version of URL
http://dx.doi.org/10.1016/j.cbpb.2006.06.008
department
Graduate School of Biosphere Science



Last 12 months's access : ? times
Last 12 months's DL: ? times


This month's access: ? times
This month's DL: ? times