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ID 14657
file
creator
Baes, Aloysius U.
Okada, Mitsumasa
subject
coagulation
purification
isolation
characterization
moringa olelfera
NDC
Pollution. Environmental engineering
description
It is known that M. oleifera contains a natural coagulant in the seeds. In our previous research, the method using salt water to extract the active coagulation component from M. oleifera seeds was developed and compared with the conventional method using water. In this research, the active coagulation component was purified from a NaCl solution crude extract of Moringa oleifera seeds. The active component was isolated and purified from the crude extract through a sequence of steps that included salting-out by dialysis, removal of lipids and carbohydrates by homogenization with acetone, and anion exchange. Specific coagulation activity of the active material increased up to 34 times more than the crude extract after the ion exchange. The active component was not the same as that of water extract. The molecular weight was about 3000 Da. The Lowry method and the phenol-sulfuric acid method indicated that the active component was neither protein nor polysaccharide. The optimum pH of the purified active component for coagulation of turbidity was pH 8 and above. Different from the conventional water extracts, the active component can be used for waters with low turbidity without increase in the dissolved organic carbon concentration.
journal title
Water Research
volume
Volume 35
issue
Issue 2
start page
405
end page
410
date of issued
2001-02
publisher
Elsevier
issn
0043-1354
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) 2000 Elsevier Science Ltd.
relation is version of URL
http://dx.doi.org/10.1016/S0043-1354(00)00290-6
department
Graduate School of Engineering



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