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ID 14652
file
creator
Shitashiro, Maiko
Tanaka, Hirohide
Hong, Chang Soo
Takiguchi, Noboru
Ohtake, Hisao
Kato, Junichi
subject
chemotaxis
chemosensory protein
trichloroethylene
methyl-acceptiong
chemotaxis protein
pseudomonas aeruginosa
NDC
Biology
description
The involvement of the chemotaxis gene cluster 1 (cheYZABW) and cheR in repellent responses of Pseudomonas aeruginosa to trichloroethylene (TCE) is described and three methyl-accepting chemotaxis proteins (MCPs) for TCE are identified. TCE chemotaxis assays of a number of deletion-insertion mutants of P. aeruginosa PAO1 revealed that the chemotaxis gene cluster 1 and cheR are required for negative chemotaxis to TCE. Mutant strains which contained deletions in pctA, pctB and pctC showed decreased responses to TCE. The pctA, pctB and pctC genes have been reported to encode MCPs for amino acids [K. Taguchi et al., Microbiology, 143, 3223–3229 (2000)]. The pctA mutation more severely impaired chemotactic responses to TCE than did those of pctB and pctC, suggesting that PctA is the major MCP for TCE among the three MCPs. The pctA, pctB and pctC mutant strains showed decreased responses to chloroform and methylthiocyanate. This result demonstrates that PctA, PctB and PctC are also involved in repellent responses to chloroform and methylthiocyanate.
journal title
Journal of Bioscience and Bioengineering
volume
Volume 99
issue
Issue 4
start page
396
end page
402
date of issued
2005-01
publisher
日本生物工学会
issn
1389-1723
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) The Society for Biotechnology, Japan
relation is version of URL
http://dx.doi.org/10.1263/jbb.99.396
department
Graduate School of Advanced Sciences of Matter



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