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ID 14208
file
creator
Tomita, Kazunori
Kibe, Tatsuya
Kang, Ho-Young
Seo, Yeon-Soo
Uritani, Masahiro
Ushimaru, Takashi
NDC
Biology
abstract
It has been suggested that the Schizosaccharomyces pombe Rad50 (Rad50-Rad32-Nbs1) complex is required for the resection of the C-rich strand at telomere ends in taz1-d cells. However, the nuclease-deficient Rad32-D25A mutant can still resect the C-rich strand, suggesting the existence of a nuclease that resects the C-rich strand. Here, we demonstrate that a taz1-d dna2-2C double mutant lost the G-rich overhang at a semipermissive temperature. The amount of G-rich overhang in S phase in the dna2-C2 mutant was lower than that in wild-type cells at the semipermissive temperature. Dna2 bound to telomere DNA in a chromatin immunoprecipitation assay. Moreover, telomere length decreased with each generation after shift of the dna2-2C mutant to the semipermissive temperature. These results suggest that Dna2 is involved in the generation of G-rich overhangs in both wild-type cells and taz1-d cells. The dna2-C2 mutant was not gamma ray sensitive at the semipermissive temperature, suggesting that the ability to process double-strand break (DSB) ends was not affected in the dna2-C2 mutant. Our results reveal that DSB ends and telomere ends are processed by different mechanisms.
journal title
Molecular and cellular biology
volume
Volume 24
start page
9557
end page
9567
date of issued
2004-11
publisher
American Society for Microbiology
issn
0270-7306
ncid
publisher doi
pubmed id
language
eng
nii type
Journal Article
HU type
Journal Articles
DCMI type
text
format
application/pdf
text version
author
rights
Copyright (c) American Society for Microbiology
relation is version of URL
http://dx.doi.org/10.1128/MCB.24.21.9557-9567.2004
department
Graduate School of Advanced Sciences of Matter



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