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ID 25
本文ファイル
著者
Uyama, Taro
Kanamori, Kan
Michibata, Hitoshi
キーワード
ascidian
metal accumulation
vacuolar H+-ATPase
vacuolre
gene expression
NDC
生物科学・一般生物学
抄録(英)
A vanadium-accumulating ascidian, Ascidia sydneiensis samea, expresses vacuolar-type H+-ATPases (V ATPases) on the vacuole membrane of the vanadium-containing blood cells known as vanadocytes. Previously, we showed that the contents of their vacuoles are extremely acidic and that a V ATPase-specific inhibitor, bafilomycin A1, neutralized the contents of the vacuoles. To understand the function of V ATPase in vanadocytes, we isolated cDNA encoding subunit C of V ATPase from vanadocytes since this subunit has been known to be responsible for the assembly of V-ATPases and to regulate the ATPase activity of V-ATPases. The cloned cDNA was 1,443 nucleotides in length, and encoded a putative 384 amino-acid protein. By expressing the ascidian cDNA for subunit C under the control of a galactose-inducible promoter, the pH-sensitive phenotype of the corresponding vma5 mutant of a budding yeast was rescued. This result showed that the ascidian cDNA for subunit C functioned in yeast cells.
掲載誌名
Marine biotechnology
3巻
開始ページ
316
終了ページ
321
出版年月日
2001
出版者
Springer
ISSN
1436-2228
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2001 Springer "The original publication is available at www.springerlink.com"
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1007/s1012601-0054-x
部局名
理学研究科