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ID 24
本文ファイル
著者
Adachi, Takahiro
Kawano, Sonoko
Aoshima, Masato
Yamaguchi, Nobuo
Kanamori, Kan
Michibata, Hitoshi
キーワード
Ascidian
Vanadium
Metal accumulation
NDC
生物科学・一般生物学
抄録(英)
Since the beginning of the last century, it has been known that ascidians accumulate high levels of a transition metal, vanadium, in their blood cells, although the mechanism for this curious biological function remains unknown. Recently, we identified three vanadium-binding proteins (vanabins), previously denoted as vanadium-associated proteins (Kanda et al., 1997), from the cytoplasm fraction of vanadium-containing blood cells (vanadocytes) of the vanadium-rich ascidian Ascidia sydneiensis samea. Here, we describe the cloning, expression, and analysis of the metal-binding ability of vanabins. Recombinant proteins of two independent but related vanabins, vanabin1 and vanabin2, bound to 10 and 20 vanadium(IV) ions with dissociation constants of 2.1 × 10-5 M and 2.3 × 10-5 M, respectively. The binding of vanadium(IV) to these vanabins was inhibited by the addition of copper(II) ions, but not by magnesium(II) or molybdate(VI) ions. Vanabins are the first proteins reported to show specific binding to vanadium ions; this should provide a clue to resolving the problem regarding the selective accumulation of vanadium in ascidians.
掲載誌名
Biochimica et biophysica acta
1626巻
開始ページ
43
終了ページ
50
出版年月日
2003
出版者
Elsevier Science B.V.
ISSN
0167-4781
出版者DOI
PubMedID
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2003 Elsevier Science B.V.
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/S0167-4781(03)00036-8
部局名
理学研究科