このエントリーをはてなブックマークに追加
ID 20
本文ファイル
著者
Yamaguchi, Nobuo
Michibata, Hitoshi
キーワード
Ascidian
Blood Cells
Chloride Channel
CLC-type
Gene Expression
H+-ATPase
Metal Accumulation
Vanadium
NDC
化学
抄録(英)
Ascidians, so-called sea squirts, can accumulate high levels of vanadium in the vacuoles of signet ring cells, which are one type of ascidian blood cell and are also called vanadocytes. In addition to containing high concentrations of vanadium in the +3 oxidation state, the proton concentrations in vanadocyte vacuoles are extremely high. In order to elucidate the entire mechanism of the accumulation and reduction of vanadium by ascidian vanadocytes, it is necessary to clarify the participation of anions, which might be involved as counter ions in the active accumulation of both vanadium and protons. We examined the chloride channel, since chloride ions are necessary for the acidification of intracellular vesicles and coexist with H+ ATPase. We cloned cDNA encoding a chloride channel from blood cells of a vanadium-rich ascidian, Ascidia sydneiensis samea. It encoded a 787-amino-acid protein, which showed striking similarity to mammalian ClC3/4/5-type chloride channels. Using a whole-mount in situ hybridization method that we developed for ascidian blood cells, the chloride channel was revealed to be transcribed in vanadocytes, suggesting its participation in the process of vanadium accumulation.
掲載誌名
Comparative biochemistry and physiology. B, Biochemistry
136巻
開始ページ
91
終了ページ
98
出版年月日
2003
出版者
Elsevier Science Inc
ISSN
1096-4959
出版者DOI
言語
英語
NII資源タイプ
学術雑誌論文
広大資料タイプ
学術雑誌論文
DCMIタイプ
text
フォーマット
application/pdf
著者版フラグ
author
権利情報
Copyright (c) 2003 Elsevier Science Inc
関連情報URL(IsVersionOf)
http://dx.doi.org/10.1016/S1096-4959(03)00175-1
部局名
理学研究科